Our antibodies to avidin and streptavidin are produced in goats using our highly purified avidin or streptavidin and isolated by affinity chromatography. Anti-Avidin does not bind streptavidin and Anti-Streptavidin does not recognize avidin. These antibodies provide opportunities to significantly amplify signals in many applications.

Biotinylated Anti-Avidin and Biotinylated Anti-Streptavidin have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding avidin or streptavidin via either biotin binding sites or through antigen binding sites, makes these biotinylated antibodies unique. These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

These affinity purified antibodies are also available unconjugated or fluorescein-labeled.

The following figure illustrates different experimental setups used to achieve increasing levels of sensitivity.