Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

The Fluorescent Streptavidin Kit contains 250 g of three fluorescently labeled streptavidin products: Fluorescein Streptavidin, Texas Red^® Streptavidin and AMCA Streptavidin. This panel of fluorescent streptavidin products provides sufficient reagent to stain 250-500 sections. Fluorescein is green-yellow (excites at 495 nm and emits at 515 nm), Texas Red^® is deep red (excites at 595 nm and emits at 615 nm) and AMCA is blue (excites at 350 nm and emits at 450 nm), providing the option of introducing multiple labels into sections.