The ImmEdge™ Pen (H-4000) is an improvement over the PAP pen for immunohistochemistry, immunocytochemistry, immunofluorescence, and in situ hybridization. It is designed to provide a heat-stable, water-repellent barrier that keeps reagents localized on tissue specimens and prevents mixing of reagents when multiple sections are mounted on the same slide.  The ImmEdge™ Pen barrier is stable for use with buffers with and without detergent (e.g. Tween 20 or Triton X-100, etc.).  

The pale blue barrier is insoluble in alcohol and acetone but is completely removed by all commonly used xylene and xylene substitute clearing agents. The ImmEdge™ Pen is compatible with enzyme or fluorescence-based detection systems.

The ImmEdge™ Pen is environmentally friendly - it does not contain "ozone-depleting" solvents as identified by the Montreal Protocol or the Council of the European Union (specifically, fluorocarbons and chlorocarbons).

Product review on Biocompare.com.