The Vector^® Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. With the aid of imaging systems and software, the spectral profile of this substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized. Vector^® Blue is also fluorescent. Sections stained with the Vector^® Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount , H-5000) must be used because the reaction product is soluble in xylene. Vector^®Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.