Inhibits endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase activities
Ready-to-use in convenient dropper bottle
Simple one-step protocol
compatible with formalin-fixed, paraffin-embedded tissue sections, frozen tissue sections, and cell preparations
More effective than conventional blocking methods
Tissues can contain endogenous peroxidase, pseudoperoxidase, and/or alkaline phosphatase activity that will produce background staining if an alkaline phosphatase and/or peroxidase detection system and corresponding substrates are used. BLOXALL™ Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution is a dual enzyme blocking reagent that inactivates endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in formalin-fixed, paraffin-embedded tissue sections, frozen tissue sections, and cell preparations in a single 10 minute incubation step. This reagent is provided as a ready-to-use reagent in a convenient dropper bottle.
Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in frozen intestine revealed with Vector Red Substrate (red) and ImmPACT DAB HRP Substrate (brown) (left. Same substrate used on BLOXALL Solution-treated tissue (right).
