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Literature

 
Literature Product Selection Guide

Product Selection Guide

[공지사항] Vector Laboratories: IF Selection Guide 2017/07/06 (10:06) 조회(452) 관리자
                                                                                                                           

 

 

Choose Primary Antibody

 

        Specific for antigen of interest

        Consider tissue species and preparation (fixation)

        Consider antigen retrieval requirements

 

 

 

 


VECTABOND™ Reagent

(Tissue Section Adhesive)

 

 

 

Blocking Reagents

Choices determined by the options selected in Steps 1-4

        Choices determined by the options selected in Steps 1-2

        Streptavidin/Biotin Blocking Kit (if using biotin/streptavidin system)

        Normal Sera (from the species of secondary antibody)

        M.O.M.™ Mouse Ig Blocking Reagent

        R.T.U. Animal-Free Block and Diluent

        BSA

        Casein Solution

 

 

 

Choose Secondary

Antibody and Tertiary

Detection System

 

        Choose fluorochrome based on wavelengths available in microscope

        Fluorochrome-conjugated secondary antibody or biotinylated secondary antibody

        Consider sensitivity requirements

        Consider species of primary antibody

        Consider tissue species

 

 

 

One Step

Single-label. Fast. Convenient  

        Fluorochrome-conjugated secondary antibodies

        VectaFluor™ R.T.U. DyLight® Labeled Secondary Antibodies

 

 

 

One Step

Dual-label two-antigen detection. Fast.

        VectaFluor™ Duet Immunofluorescence Double Labeling Kits

 

 

 

 

 

Two Step

Biotin-based.

        Biotinylated secondary antibody and fluorescent avidin or streptavidin Fluorochrome conjugated.

        See Step 3 for additional amplification

 

 

 

 

 

 

 

Two Step

Highest sensitivity. Non-biotin based.

        VectaFluor™ Excel Amplified Fluorescent Staining System (Amplifier Antibody + fluorescent tertiary antibody)

 

 

Choose Signal Amplication with Biotin-based Systems

(Option C)


        Multiple rounds of amplification possible (with biotin-based systems)

 

 

 

 

 

 

 

Choose Nuclear Counterstain


        VECTASHIELD® antifade mounting media, with or without counterstain

 

 

 

 

 

 

 

Choose Mounting Media


        Fluorescence microscope

        View using appropriate excitation/emission filters

 

Prostate: Anti-PSA (goat), VectaFluor™ DyLight® 488 Anti-Goat IgG. Mounted in VECTASHIELD® HardSet™ Mounting Medium.




 

 

 

Ordering Information

 

Tissue Preparation

 

Fluorochrome-Labeled Secondary Antibodies

 

 

Secondary Antibody Kits

Product

Catalog #

 

Product

AMCA

Fluorescein

DyLight® 488

DyLight® 549

DyLight® 594

DyLight® 649

Cy3

Cy5

 

Product

Catalog #

VECTABOND™ Reagent (Tissue Section Adhesive)

SP-1800

 

Anti-Mouse IgG (H+L), made in horse

CI-2000

FI-2000

DI-2488

DI-2549

DI-2594

DI-2649

CY-2300

CY-2500

 

VectaFluor™ Duet Kits

 

Anti-Mouse IgG (H+L), rat adsorbed, made in horse

 

FI-2001

 

 

 

 

 


 

VectaFluor™ Duet IF Double Labeling Kit

- DyLight® 488 Anti-Rabbit (green)

- DyLight® 594 Anti-Mouse (red)

DK-8818




ImmPrint™ Histology Pen

H-6100

 

Anti-Mouse IgM, mu chain specific, made in goat


FI-2020







 

ImmEdge™ Hydrophobic Barrier Pen

H-4000

 

Anti-Rabbit IgG (H+L), made in horse



DI-1088

DI-1094





 

 

 

 

Anti-Rabbit IgG (H+L), made in goat

CI-1000

FI-1000

DI-1488

DI-1549

DI-1594

DI-1649

CY-1300

CY-1500

 

VectaFluor™ Duet IF Double Labeling Kit

- DyLight® 594 Anti-Rabbit (red)

- DyLight® 488 Anti-Mouse (green)

DK-8828

Antigen Retrieval

 

Anti-Rat IgG (H+L), made in rabbit

FI-4000








 

Product

Catalog #

 

Anti-Rat IgG (H+L), mouse adsorbed, made in rabbit

FI-4001








 

Antigen Unmasking Solution Citrate-based (100X) (pH 6.0)

H-3300

 

Anti-Rat IgG, made in goat






CY-4300

CY-4500


 

Mouse on Mouse (M.O.M.) Kits

 

Anti-Goat IgG (H+L), made in horse


DI-3088


DI-3094





 

M.O.M.™ Basic Kit

BMK-2202

Antigen Unmasking Solution Tris-based (100X) (pH 9.0)

H-3301

 

Anti-Goat IgG (H+L), made in rabbit

CI-5000

FI-5000







 

M.O.M.™ Fluorescein Kit

FMK-2201

 

VectaFluor™ Anti-Rabbit IgG, made in horse


DI-1788


DI-1794





 

 

 

Quench/Block

Catalog #

 

VectaFluor™ Anti-Mouse IgG, made in horse


DI-2788


DI-2794





 

Counterstain/Mount


Quench/Block

 

VectaFluor™ Anti-Goat IgG, made in horse


DI-3788


DI-3794





 

Product

Catalog

Product

Catalog #

 

Anti-Human Fluorochrome-Labeled Secondary Antibodies

 

Non-Hardening Formula

BLOXALL™ Endogenous HRP and AP Blocking Solution

SP-6000

 

Anti-Human IgG (H+L), made in goat


FI-3000

 

 



 

 

 

VECTASHIELD® Mounting Medium

H-1000

Avidin/Biotin Blocking Kit

SP-2001

 

Anti-Human IgE, epsilon chain specific, made in goat


FI-3040

 

 



 

 

 

VECTASHIELD® Mounting Medium with DAPI

H-1200

Streptavidin/Biotin Blocking Kit

SP-2002

 

Anti-Human IgG, gamma chain specific, made in goat


FI-3080

 

 


 

 

 

 

VECTASHIELD® Mounting Medium with PI

H-1300

Normal Goat Serum

S-1000

 

Anti-Human IgM, mu chain specific, made in goat


FI-3020

 

 


 

 

 

 

Hardening Formula

Normal Horse Serum

S-2000

 

Anti-Human Kappa Chain, made in goat

CI-3060

FI-3060

 

 


 

 

 

 

VECTASHIELD® HardSet™ Mounting Medium

H-1400

Normal Rabbit Serum

S-5000

 

Anti-Human Lambda Chain, made in goat

CI-3070

FI-3070

 

 


 




VECTASHIELD® HardSet™ Mounting Medium with DAPI

H-1500

2.5% Normal Horse Serum

S-2012

 



Bovine Serum Albumin (BSA)

SP-5050

 

Tertiary Reagents

 


VECTASHIELD® HardSet™ Mounting

Medium with TRITC-Phalloidin

H-1600

10x Casein Solution

SP-5020

 

Product

Biotin

AMCA

Fluorescein

DyLight® 488

DyLight® 549

DyLight® 594

DyLight® 649

Cy3

Cy5


R.T.U. Animal-Free Block and Diluent

SP-5035

 

VectaFluor™ Excel Amplified Anti-Rabbit IgG Kit

 

 

 

DK-1488

 

DK-1594

 

 

 


 

 

M.O.M.™ Mouse Ig Blocking Reagent

MKB-2213

 

VectaFluor™ Excel Amplified Anti-Mouse IgG Kit

 

 

 

DK-2488

 

DK-2594

 

 

 


 


Carbo-Free™ Blocking Solution

SP-5040

 

Streptavidin

 

SA-5008

SA-5001

SA-5488

SA-5549

SA-5594

SA-5649

SA-1300

SA-1500


 

 

 

 

 

Avidin

 

A-2008

A-2001

 

 

 

 

 

 

 

 

 

 

 

 

Avidin DCS

 

 

A-2011

 

 

 

 

 

 

 

 

 

 

 

 

Anti-Streptavidin

BA-0500

 

 

SP-4488

SP-4549

 

 

 

 

 

 

 

 

 

 

Anti-Avidin

BA-0300