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Literature

 
Literature Product Selection Guide

Product Selection Guide

[공지사항] Vector Laboratories: IHC Selection Guide 2019/08/30 (14:02) 조회(1453) 관리자


 

Choose Primary Antibody

 

        Specific for antigen of interest

        Consider tissue species and preparation (fixation)

        Consider antigen retrieval requirements

 


VECTABOND™ Reagent

(Tissue Section Adhesive)

 

 

Blocking Reagents

Choices determined by the options selected in Steps 1-4

        BLOXALL™ endogenous enzyme blocking reagents (HRP/AP)


        Avidin/Biotin Blocking Kit

(if using VECTASTAIN® ABC system)

 

        Normal Sera 

         (from the species of secondary antibody)

 

        M.O.M.™ Mouse Ig Blocking Reagent


        Animal-Free Block and Diluent


        BSA


        Casein Solution

 

 


 

Choose Enzyme Substrate

 

        Color

        Compatibility with other system reagents (counterstains, mounting media and other substrates for multiplexing)


 

 


 

Choose Secondary

Antibody and Tertiary

Detection System

 

        Choose HRP or AP

enzyme system

        Consider sensitivity requirements

        Consider species of primary antibody

        Consider tissue species


 

 

One Step

Convenient. Consistent. Ready-to-use. Non-Biotin based.

        ImmPRESS™ Polymer Reagents

 


 

 


 

Choose Nuclear Counterstain

 

        Blue, green, or red

        Compatibility with substrate, mounting media


 

 


 

 

Two Step

Economical. Biotin-based.

        Biotinylated secondary antibody

+ ABC Complex (VECTASTAIN® Elite® ABC Kit)

 


 

 


 

Choose Mounting Media

 

        Aqueous vs. non-aqueous

        Compatibility with substrate(s) and counterstain


 

 


 

 

Two Step

Highest sensitivity. Non-biotin based.

        ImmPRESS™ Excel Amplified Polymer Staining Systems

 



 

Visualize

 

       Brightfield microscope


 

Small bowel: CD10 (m), VECTASTAIN® Elite® ABC Kit, ImmPACT™ DAB HRP Substrate (brown). Hematoxylin QS counterstain (blue).

 


 

 

 

Tissue Preparation


 

Secondary Antibodies and Tertiary Reagents


 

Substrates/Chromogen


Product

Catalog #

 

Product

Catalog #

 

Product

Catalog #

 

Product

Catalog #

VECTABOND™ Reagent (Tissue

Section Adhesive)

SP-1800

 

ImmPRESS™ Polymer Reagents

 

VECTASTAIN® ABC Complex Reagents

 

Vector® DAB

SK-4100

ImmPrint™ Histology Pen

H-6100

 

ImmPRESS™ HRP Anti-Rabbit IgG Kit (made in horse)

MP-7401

 

VECTASTAIN® Elite® ABC Kit, Standard, HRP

PK-6100

 

Vector® DAB +Ni

SK-4100

ImmEdge™ Hydrophobic Barrier Pen

H-4000

 

ImmPRESS™ HRP Anti-Rabbit IgG Kit (made in goat)

MP-7451

 

VECTASTAIN® Elite® ABC Kit, Rabbit IgG, HRP

PK-6101

 

ImmPACT™ DAB

SK-4105

 

 

 

ImmPRESS™ HRP Anti-Mouse IgG Kit, (made in horse)

MP-7402

 

VECTASTAIN® Elite® ABC Kit, Mouse IgG, HRP

PK-6102

 

ImmPACT™ DAB EqV

SK-4103

Antigen Retrieval

 

ImmPRESS™ HRP Anti-Mouse IgG Kit (made in goat)

MP-7452

 

VECTASTAIN® Elite® ABC Kit, Rat IgG, HRP

PK-6104

 

Vector® VIP

SK-4600

Product

Catalog #

 

ImmPRESS™ HRP Anti-Mouse IgG, Rat Adsorbed, Kit (made in horse)

MP-7422

 

VECTASTAIN® Elite® ABC Kit, Goat IgG, HRP

PK-6105

 

Vector® SG

SK-4700

Antigen Unmasking Solution

Citrate-based (100X) (pH 6.0)

H-3300

 

ImmPRESS™ HRP Anti-Rat IgG Kit (made in horse)

MP-7404

 

VECTASTAIN® Elite® ABC Kit, Universal, HRP

PK-6200

 

Vector® NovaRED™

SK-4800

Antigen Unmasking Solution

Tris-based (100X) (pH 9.0)

H-3301

 

ImmPRESS™ HRP Anti-Rat IgG Kit, Mouse Adsorbed Kit (made in horse)

MP-7444

 

R.T.U. VECTASTAIN® Elite® ABC Reagent, HRP

PK-7100

 

ImmPACT™ NovaRED™

SK-4805

 

 

 

ImmPRESS™ HRP Anti-Goat IgG Kit (made in horse)

MP-7405

 

R.T.U. VECTASTAIN® Elite® ABC Kit, Universal, HRP

PK-7200

 

Vector® AEC

SK-4200

Quench/Block

 

ImmPRESS™ HRP Universal Antibody Kit, Anti-Rabbit/ Mouse Kit (made in horse)

MP-7500

 

VECTASTAIN® ABC Kit, Standard, AP

AK-5000

 

ImmPACT™ AEC

SK-4205

Product

Catalog #

 

ImmPRESS™ VR Anti-Rabbit IgG Kit (made in horse)

MP-6401

 

VECTASTAIN® ABC Kit, Rabbit IgG, AP

AK-5001

 

ImmPACT™ AMEC Red

SK-4285

BLOXALL™ Endogenous HRP and AP Blocking Solution

SP-6000

 

ImmPRESS™ VR Anti-Rabbit IgG Kit (made in horse)

MP-6402

 

VECTASTAIN® ABC Kit, Mouse IgG, AP

AK-5002

 

TMB

SK-4400

Avidin/Biotin Blocking Kit

SP-2001

 

ImmPRESS™ Excel Amplified Anti-Rabbit IgG Kit (made in horse)

 

MP-7601

 

VECTASTAIN® ABC Kit, Universal

AK-5200

 

Vector® Red

SK-5100

Streptavidin/Biotin Blocking Kit

SP-2002

 

ImmPRESS™ Excel Amplified Anti-Mouse IgG Kit (made in horse)

MP-7602

 

Biotinylated Secondary Reagents

 

ImmPACT™ Vector® Red

SK-5105

Normal Goat Serum

S-1000

 

ImmPRESS™ AP Anti-Rabbit IgG Kit (made in horse)

MP-5401

 

Anti-Goat IgG (H+L) made in rabbit

BA-5000

 

Vector® Blue

SK-5300

Normal Horse Serum

S-2000

 

ImmPRESS™ AP Anti-Mouse IgG Kit (made in horse)

MP-5402

 

Anti-Goat IgG (H+L) made in horse*

BA-9500

 

BCIP/NBT

SK-5400

Normal Rabbit Serum

S-5000

 

ImmPRESS™ AP Anti-Rat IgG Kit (made in horse)

MP-5404

 

Anti-Human IgG (H+L) made in goat

BA-3000

 

 

 

2.5% Normal Horse Serum

S-2012

 

ImmPRESS™ AP Anti-Goat IgG Kit (made in horse)

MP-5444

 

Anti-Mouse IgG (H+L) made in horse*

BA-2000

 

Counterstain


Bovine Serum Albumin (BSA)

SP-5050

 

ImmPRESS™ AP Anti-Goat IgG Kit (made in horse)

MP-5405

 

Anti-Mouse IgG (H+L) made in horse, rat adsorbed

BA-2001

 

Product

Catalog #

10x Casein Solution

SP-5020

 

Mouse on Mouse  (M.O.M.) Kits

 

Anti-Mouse IgG (H+L) made in goat*

BA-9200

 

Vector® Hematoxylin

H-3401

R.T.U. Animal-Free Block and Diluent

SP-5035

 

M.O.M.™ ImmPRESS™ HRP Polymer Kit

MP-2400

 

Anti-Mouse IgM (H+L) μ chain specific, made in goat

BA-2020

 

Vector® Hematoxylin QS

H-3404

M.O.M.™ Mouse Ig Blocking Reagent

MKB-2213

 

M.O.M.™ Basic Kit

BMK-2202

 

Anti-Rabbit IgG (H+L) made in goat*

BA-1000

 

Vector® Methyl Green

H-3402

Carbo-Free™ Blocking Solution

SP-5040

 

M.O.M.™ Peroxidase Kit

PK-2200

 

Anti-Rat IgG (H+L) made in rabbit

BA-4000

 

Vector® Nuclear Fast Red

H-3403

 

 

 

M.O.M.™ Biotinylated Anti-Mouse Ig Reagent

MKB-2225

 

Anti-Rat IgG (H+L) made in rabbit, mouse adsorbed

BA-4001

 

 

 

 

 

 

M.O.M.™ ImmPRESS™ HRP Polymer Anti-Mouse Reagent

MPX-2402

 

Anti-Rat IgG (H+L) made in goat*

BA-9400

 

Coverslip/Mount

 

 

 

 

 

 

Universal Anti-Mouse/Rabbit IgG (H+L) made in horse*

BA-1400

 

Product

Catalog #

 

 

 

 

 

 

 

 

 

VectaMount™ Permanent Mounting Medium

H-5000

 

 

 

 

 

 

 

 

 

VectaMount™ AQ Aqueous Mounting Medium

H-5501